A CT image of a mouse injected with 40 mg gold nanoparticles (15nm) is presented. The whole body CT image was acquired 1 hour post injection in 15 minutes with 45 kvP tube voltage and 1000ms exposure time.

A focused scan of just the upper torso of the same animal is available here.

The data was kindly provided by Ciara Finucane.

Brain image analysis of non-human primates may be greatly aided through the use of anatomical atlases.  This animated GIF shows a surface rendering of one hemisphere of inviCRO's 15-region rhesus macaque brain atlas.

Visualization of a mouse atlas in inviCRO's new 3D ROI tool. This atlas is used by InVivoScope and VivoQuant to visualize bio distribution data and contains 24 regions.

Fused Whole Body Bone CT scan (55kVp, 1100ms, 117µm voxel size) and Fluorine Whole body PET (20min scan, 50µCi 18Fluorine, 45min PI).

Whole body bone CT scan (55kVp, 1100ms, 142µm voxel size)

We previously assessed the efficiency of intravenous injections of 125I-labeled mAbs for the treatment of small peritoneal carcinomatosis (Santoro et al, J. Nucl Med 2009). In the present study, athymic nude mice bearing intraperitoneal A-431 tumor cell xenografts expressing CEA receptors were intraperitoneally injected with 125I-35A7 mAb at days 4 following tumor cells xenograft. SPECT-CT imaging shows strong uptake of 125I-mAbs by tumors nodules, and demonstrates its usefulness for radioimmunotherapy of small solid tumors.
Tracer details : The non-internalizing murine IgG1k 35A7 mAb, specific for the CEA Gold 2 epitope, was used to target CEA in transfected A-431 cells. Radiolabeling with iodine 125 was done according to the IODO-GEN method.

Contributor : Jean-Pierre Pouget, IRCM

A quadruple isotope SPECT/CT imaging acquisition was performed on a rat. 450uCi (17MBq) of Tc^99m-MDP, 1.4mCi (52MBq) of I¹²³, 600uCi (22MBq) of In¹¹¹, and 1mCi (37MBq) of Tl²⁰¹-Chloride were administered to the rat. A 40-minute image acquisition was performed at 3 hours post-injection of the Tc^99m/I¹²³ and 1 hour post-injection of the In¹¹¹/Tl²⁰¹.

The color bars from left to right are: CT, Tc^99m, I¹²³, In¹¹¹ and Tl²⁰¹.

Please see [158] for more details.

Data courtesy of the University of New Mexico College of Pharmacy.

A cohort of ferrets was induced with the flu in an attempt to visualize uptake of radiotracer in the sinuses. In this trial experiment 590uCi of In-111 (green) labeled with an inflammation targeting agent were injected. A fast, 7-minute CT acquisition was acquired over a 230mm scan range followed by a 20-minute SPECT acquisition. In-111 uptake in the trachea, heart, and lymphatic regions is clearly visible. Note also the striations visible in the ferret's trachea.

Images courtesy of the University of New Mexico, College of Pharmacy, and the Lovelace Respiratory Research Institute.

Dual-isotope ECG-gated SPECT/CT imaging was performed on a rat. 800uCi (30MBq) of Tc^99m-SestaMIBI and 970uCi (36MBq) of Tl²⁰¹-Chloride were injected into a rat. Data were acquired for 45 minutes at 60 minutes post-injection. Average heart rate was stable at ~320 BPM with the rat held under gas anesthesia. The image presents Tc^99m+CT data on the left and Tl²⁰¹+CT data on the right.

Images courtesy of the University of New Mexico.

A quadruple isotope SPECT/CT imaging acquisition was performed on a rat. 450uCi (17MBq) of Tc^99m-MDP, 1.4mCi (52MBq) of I¹²³, 600uCi (22MBq) of In¹¹¹, and 1mCi (37MBq) of Tl²⁰¹-Chloride were administered to the rat. A 40-minute image acquisition was performed at 3 hours post-injection of the Tc^99m/I¹²³ and 1 hour post-injection of the In¹¹¹/Tl²⁰¹. From left to right, the image displays reconstructions of Tc^99m (gray; bone uptake), I¹²³ (purple; thyroid and small intestine uptake), In¹¹¹ (blue; heart, liver, and intestine uptake), and Tl²⁰¹ (red; kidney, liver, and heart uptake).

Images courtesy of the University of New Mexico College of Pharmacy.

Displayed is high-resolution ex vivo image of microcalcification of breast cancer. A CT scan was performed with tube voltage of 45 kVp, 177µA current, 3000 ms/projection, 360 projections/rotation. The pixel size of detector was set to 96 µm. This image was acquired in 18 minutes. Exact cone beam algorithm with a Shepp-Logan filter and 10-micron voxel size was used for reconstruction (using InVivoScope post-processing software).

The image is courtesy the Frangioni Laboratory, Longwood SAIF, Harvard Medical school.

SPECT/CT imaging of a mouse was performed following administration of three isotopes. 700uCi (26MBq) of Tc^99m-MDP, 1.2mCi (44MBq) of I¹²³, and 300uCi (11MBq) of Tl²⁰¹ were injected. Imaging was performed for 56 minutes at 60 minutes post-injection. MDP bone uptake is visualized in gray, I¹²³ uptake (red) is clearly visible in the thyroid, small intestine, and eyes, while Tl²⁰¹ (green) uptake is mostly evident in the kidneys.

Images courtesy of The University of New Mexico College of Pharmacy.

The results of an automated tumor segmentation of a mouse using inviCRO's digital scalpel. The layered model or "onion" model is generated via topographical thinning or "peeling" of the segmented tumor.
The four scenes are comprised of (left-to-right) an X-Ray CT (used to segment the tumor), the CT plus related SPECT data of the entire animal, the CT plus SPECT of the segmented tumor only (different color scale), and the CT plus inviCRO's onion model of the segmented tumor.
This model, applicable to various imaging fields (see [148]), allows to analyze uptake within an organ.

Special thanks to Ben Gershman, University of New Mexico and Dr. Jacob Hesterman, Bioscan for their support.

A three-part animation displaying the results of an automated lung segmentation of a rat using the newly developed inviCRO digital scalpel. The layered model or "onion" model of the lung is then generated via topographical thinning or "peeling" of the segmented lung. Application of this model to the image data allows for automated, quantifiable deposition of the relevant radiopharmaceutical in the lung. The three scenes are comprised of an X-Ray CT/Onion model fusion, SPECT/CT and lastly SPECT/Onion model.

Exemplary segmentation of a mouse brain using the CT component of a NanoSPECT/CT image only. On the left hand side, the original CT slices are shown, while the right images displays a fusion with the four different classifications of this image (background=gray, body=red, bone=white, brain=yellow).

Such classified anatomical data can subsequently be used to isolate organs from other modalities and thus achieve very precise and reliable information on, for instance, uptake.
See [141] for further details.

InviCRO offers tools for automated segmentation for this and many other applications.

Approximately 150uCi (in 50uL) of stannous colloids labeled with Tc-99m were nebulized into the lungs of a normal FVB mouse with a microsprayer. Image data were acquired using a high-resolution, whole body multi-pinhole (9) aperture over 18 minutes, beginning approximately 15 minutes post-administration. A fully 3D volume rendering of the SPECT/CT data is shown.

Images courtesy of Alain Le Pape, CNRS, Orleans, France

Example of an automated tumor segmentation in a mouse using Definiens' Developer XD software.
On the left, a volume rendering of the mouse torso (CT in grayscale, SPECT in NIH Fire 2) is shown, while on the right hand side, the tumor was isolated using inviCRO's digital scalpel tool.

The segmentation rule set provided by the Boston-based contract research organization finds the tumor based on its characteristics in the anatomical CT only. This approach not only allows a very precise volume measurement (including necrotic parts of the tumor), but also delivers highly consistent estimates of the tumor uptake from the SPECT image. SPECT and CT images generated on Bioscan's NanoSPECT/CT.

Please note, that different SPECT color scales where used in the left and right movie.

Special thanks to Dr. Hesterman (Bioscan) for his support in data pre-processing and image generation. The mouse image is courtesy of Ben Gershman (Univ. New Mexico).

To analyze the aerosol images shown in [131] and [133], inviCRO developed a segmentation rule set for Definiens' Developer XD software. This does not only allow for very precise and consistent segmentation of a single image, but can easily be used for batch-processing of an array of images.
The CT-only based segmentation delivers valuable anatomical information to analyze the information gained from the SPECT scans. To isolate the lungs from the rest of the image, inviCRO used their digital scalpel.

Special thanks to Ky Harlin (inviCRO, LLC) for the image processing. For additional details on the mouse data, please see above links.

A tri-modality (grayscale MRI, green SPECT, NIH Fire2 CT) MIP of a rat brain acquired by inviCRO at the Center for Translational Neuroimaging in the newly created imaging facility at Northeastern University.

A rule set (written in the Definiens Developer package) was applied to the CT data to automatically segment the brain and bones. The resulting segmentation was used in inviCRO's digital scalpel to isolate brain from the MR and SPECT data with anatomical reference supplied by the bone CT data.

The MRI (100umx100umx500um, 30min acq.) was acquired on a 7T Bruker magnet using a spin-echo sequence (RARE) comprised of 50 slices with 6 averages. The SPECT (600um voxels, 30min acq.) and CT (400um voxels, 4.5min acq.) images were generated on a NanoSPECT/CT. 400 uCi of I125-beta-CIT (a dopamine and serotonin transporter imaging agent) was injected 4.5 hours prior to the SPECT study.

After this process, the image data is now ready for futher analysis, such as estimating uptake in different regions of the brain.

The unsegmented data can be found in [139] and [138].

Special thanks to Dr. Hesterman (Bioscan) for the data processing.

Transverse slices from a tri-modality study (MRI, SPECT, CT) of a rat brain acquired at the Center for Translational Neuroimaging in the newly created imaging facility at Northeastern University. The MRI (grayscale, 100umx100umx500um, 30min acq.) was acquired on a 7T Bruker magnet using a spin-echo sequence (RARE) comprised of 50 slices with 6 averages. The SPECT (green, 600um voxels, 30min acq.) and CT (NIH Fire2, 400um voxels, 4.5min acq.) images were generated on a NanoSPECT/CT. 400 uCi of I125-beta-CIT (a dopamine and serotonin transporter imaging agent) was injected 4.5 hours prior to the SPECT study. Tracer localized in eyes, striatum and thyroid.
Animal was imaged on a Minerve bed under isoflurane. Data registration and presentation supported by inviCRO.